Journal: PLoS ONE

Article Title: Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck

doi: 10.1371/journal.pone.0271746

Figure Lengend Snippet: Immunization plan in the experimental ducks.

Article Snippet: MoDCs were then stained with Rabbit FITC-H9N2 HA protein monoclonal antibody (Sino Biological Inc. Beijing, China) at 4°C for 1 h. Then washed twice with PBS, and re-suspended in 4% paraformaldehyde for flow cytometry (FACSAria III, BD Biosciences).

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck

doi: 10.1371/journal.pone.0271746

Figure Lengend Snippet: (A) Representative flow cytometry profile of the positive rates of MoDCs catching H9N2 IAIV antigen. From group a to group h are RPMI-1640 mock control group, H9N2 IAIV group, pam2CSK4-H9N2 IAIV group, poly (I:C)-H9N2 IAIV group, imiquimod-H9N2 IAIV group, pam2CSK4-imiquimod-H9N2 IAIV group, poly (I:C)-imiquimod-H9N2 IAIV group and pam2CSK4-poly (I:C)-imiquimod-H9N2 IAIV group, respectively. The data presented here are results from one experiment of three flow cytometry experiments. (B) The statistical graph of the positive rates of MoDCs catching H9N2 IAIV antigen. The data were analyzed using Flowjo7.6 software. Data represent the mean ±SEM of three independent experiments. Different letters (a~c) mean significant difference ( P <0.05); ab means has no significant difference between adjacent a or b ( P >0.05).

Article Snippet: MoDCs were then stained with Rabbit FITC-H9N2 HA protein monoclonal antibody (Sino Biological Inc. Beijing, China) at 4°C for 1 h. Then washed twice with PBS, and re-suspended in 4% paraformaldehyde for flow cytometry (FACSAria III, BD Biosciences).

Techniques: Flow Cytometry, Software

Journal: Influenza and Other Respiratory Viruses

Article Title: SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus

doi: 10.1111/irv.13114

Figure Lengend Snippet: Schematic of the IMBSIs@Ag‐SERS method for H5N1 influenza virus detection.

Article Snippet: Then, 1 μg/ml, 0.5 μg/ml, 0.33 μg/ml, 0.25 μg/ml and 0.1 μg/ml of biotinylated rabbit anti‐H5N1 antibody (Sino Biological Inc., Beijing, China) were added to the MB‐SA and incubated for 1 h at room temperature with gentle rotation in the tube.

Techniques:

Journal: Influenza and Other Respiratory Viruses

Article Title: SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus

doi: 10.1111/irv.13114

Figure Lengend Snippet: Detection of H5N1 influenza virus by IMBSIs@Ag‐SERS method. (A) Effect of SERS enhancement in situ reduction of nano‐silver. (B) SERS of H5N1 influenza virus captured by the primary antibodies in different concentrations. (C) TEM of naked magnetic beads with streptavidin in situ reduction of nano‐silver. (D) TEM of immunomagnetic beads sandwich immunocomplexes (IMBSIs) in situ reduction of nano‐silver.

Article Snippet: Then, 1 μg/ml, 0.5 μg/ml, 0.33 μg/ml, 0.25 μg/ml and 0.1 μg/ml of biotinylated rabbit anti‐H5N1 antibody (Sino Biological Inc., Beijing, China) were added to the MB‐SA and incubated for 1 h at room temperature with gentle rotation in the tube.

Techniques: In Situ, Magnetic Beads

Journal: Influenza and Other Respiratory Viruses

Article Title: SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus

doi: 10.1111/irv.13114

Figure Lengend Snippet: Excellent repeatability and specificity of SERS detection in H5N1 influenza virus. (A) The IMBSIs@Ag SERS showed a consistent Raman intensity at 1053 cm −1 for nine times. (B) The IMBSIs@Ag SERS no characteristic peak at 1053 cm −1 replacing H5N1 with H1N1, H5N6 or H9N2.

Article Snippet: Then, 1 μg/ml, 0.5 μg/ml, 0.33 μg/ml, 0.25 μg/ml and 0.1 μg/ml of biotinylated rabbit anti‐H5N1 antibody (Sino Biological Inc., Beijing, China) were added to the MB‐SA and incubated for 1 h at room temperature with gentle rotation in the tube.

Techniques:

Journal: Influenza and Other Respiratory Viruses

Article Title: SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus

doi: 10.1111/irv.13114

Figure Lengend Snippet: The susceptibility of IMBSIs@Ag for detection of the H5N1 influenza virus. (A) IMBSIs@Ag Raman intensity of the H5N1 influenza virus was 10‐fold diluted from 5.0 × 10 6 to 5.0 × 10 −7 TCID 50 /ml. (B) Linear relationship between Raman intensity and H5N1 influenza virus concentration. (C) IMBSIs@Ag SERS identification of H5N1 influenza virus from chicken embryos.

Article Snippet: Then, 1 μg/ml, 0.5 μg/ml, 0.33 μg/ml, 0.25 μg/ml and 0.1 μg/ml of biotinylated rabbit anti‐H5N1 antibody (Sino Biological Inc., Beijing, China) were added to the MB‐SA and incubated for 1 h at room temperature with gentle rotation in the tube.

Techniques: Concentration Assay

Journal: Experimental Biology and Medicine

Article Title: Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates

doi: 10.1177/15353702221113860

Figure Lengend Snippet: GC-FP-based detection of Nuc protein using MM05 as the capture antibody and R019 as the detection antibody. Positive controls included Nuc protein and R019 antibodies directly immobilized on the GC-FP chip. Negative controls included human IgG and CoV-2 S1S2 (spike) protein. One-way ANOVA followed by Tukey’s multiple comparison test were performed using these data. *** P = 0.0004. **** P < 0.0001.

Article Snippet: Nucleocapsid (Nuc) protein, the S1 fragment of the spike protein (S1), the extracellular domain of the spike protein (S1S2), human serum albumin (HSA), MM05 monoclonal mouse anti-Nuc antibody (MM05), MM08 monoclonal mouse anti-Nuc antibody (MM08), R019 monoclonal rabbit anti-Nuc antibody (R019), and R004 monoclonal rabbit anti-Nuc antibody (R004) were all obtained from Sino Biological (Wayne, PA), Inc.

Techniques:

Journal: Experimental Biology and Medicine

Article Title: Dual detection of COVID-19 antigens and antibodies using nanoscale fluorescent plasmonic substrates

doi: 10.1177/15353702221113860

Figure Lengend Snippet: Evaluation of capture and detection antibody pairs on V22 chips for direct detection of CoV-2 Nuc protein.

Article Snippet: Nucleocapsid (Nuc) protein, the S1 fragment of the spike protein (S1), the extracellular domain of the spike protein (S1S2), human serum albumin (HSA), MM05 monoclonal mouse anti-Nuc antibody (MM05), MM08 monoclonal mouse anti-Nuc antibody (MM08), R019 monoclonal rabbit anti-Nuc antibody (R019), and R004 monoclonal rabbit anti-Nuc antibody (R004) were all obtained from Sino Biological (Wayne, PA), Inc.

Techniques: Negative Control